Silica-based Aqueous SEC
Silica-based Aqueous SEC Columns from Shodex
- Network or pores on the surface of the packing material works as molecular sieve to separate molecules based on their sizes
- To separate molecules solely based on their sizes, it requires an analytical condition without any analyte and packing gel interaction
- The bigger the molecule size, the faster the elution sequence
- Used for molecular weight or molecular distribution determination of macromolecules and qualification of oligomers
All pictures shown are for illustration purpose only. Actual product may vary.
- Buy
- Features
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KW-800 - Silica-based packed columns for aqueous SEC analysis
- Suitable for the analysis of proteins and enzymes
- Fulfills USP L20, L33 and L59 requirements
LW-803 - Suitable for pore size specifically controlled for analysing proteins with molecular weights of several hundred thousand
- High performance analysis of antibody drugs and various proteins
- High lot-to-lot reproducibility
- Fulfills USP L20, L33 and L59 requirements
KW400 - Reduced packing material particle size enhances column performance
- Three to four-fold higher sensitivity than KW-800 series
- KW405-F is applicable analysing samples with molecular weight above 1,000,000
- Fulfills USP L20, L33 and L59 requirements
- Specifications
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Target molecular weight range and exclusion limit
Use the below tables for reference purposes only when selecting columns.
Measured with protein (eluent: phosphate buffer)
Name Target Molecular Weight Range Exclusion Limit KW-802.5
5000 - 100,000 150,000 KW-803, LW-803
10,000 - 700,000 *(1,000,000) KW-804
30,000 - *(4,000,000) *(4,000,000) KW402.5
5000 - 70,000 150,000 KW403
10,000 - 500,000 600,000 KW404
30,000 - *(4,000,000) *(4,000,000) KW405
200,000 - *(20,000,000) *(20,000,000) *( ) Estimated value
Measured with pullulan (eluent: ultrapure water)
Name Target Molecular Weight Range Exclusion Limit KW-802.5
2000 - 50,000 60,000 KW-803
5000 - 100,000 170,000 KW-804
20,000 - 300,000 500,000 KW402.5
2000 - 40,000 60,000 KW403
3000 - 50,000 80,000 KW404
20,000 - 300,000 400,000 KW405
100,000 - 700,000 1,300,000